MJSTEM

Abstract

The study investigates the antimicrobial activity of methanolic fraction ME of ethyl acetate extract derived from honeycomb against Typhoid and Paratyphoid bacilli, the pathogens responsible for typhoid and paratyphoid fever. Given the rising concern regarding antimicrobial resistance (AMR) among Salmonella strains, this research aims to explore alternative therapeutic approaches utilizing natural products. The methodology encompasses collecting and preparing honeycomb samples, followed by extraction, concentration, and antimicrobial susceptibility testing against standardized bacterial strains. Results indicated that the methanolic fraction of ethyl acetate extract exhibits significant antibacterial properties, potentially inhibiting bacterial growth effectively at 2000µg/cm3. The Minimum inhibitory Concentration (MIC) and Minimum Bacteriocidal Concentration (MBC) of the fractions resistance to Salmonella typhi and paratyphi was at 2000µg/cm3. Mechanistic studies utilizing microscopy techniques provide insights into the morphological changes induced by the methanolic fraction of ethyl acetate extract on bacterial cells. Statistical analyses confirm the robustness and significance of the findings. Hence, this research underscores the potential of honeycomb extracts as adjunctive therapies in managing enteric infections, contributing to the search for novel antimicrobial agents in the face of escalating AMR challenges.

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